DNA

Part:BBa_M34014:Design

Designed by: Kristine Kim   Group: MIT BE 20.20 - S11   (2011-05-05)


Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1980
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1980
    Illegal BglII site found at 96
    Illegal BglII site found at 2482
    Illegal BglII site found at 2538
    Illegal BamHI site found at 130
    Illegal BamHI site found at 264
    Illegal BamHI site found at 1720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1980
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1369


Design Notes

Since it is a copy of a cDNA derived from RNA that is already spliced, the sequence only reflects the exon parts, not the introns.


Source

It comes from the cDNA corresponding to the RNA of the bullfrog (Rana Catesbeiana) liver.

References